- Your Product Type
- Your Study Type
- Aquatic Ecotoxicology
- Aquatic Invertebrates
- OECD 202: Daphnia sp., Acute Immobilisation Test
- OECD 211: Daphnia magna Reproduction Test
- OECD 235: Chironomus sp., Acute Immobilisation Test
- OECD 218/219: Sediment-Water Chironomid Toxicity Test Using Spiked Sediment/Spiked Water
- OECD 233: Sediment-Water Chironomid Life-Cycle Toxicity Test Using Spiked Water or Spiked Sediment
- OECD 225: Sediment-water Lumbriculus Toxicity Test Using Spiked Sediment
- OECD 242: Potamopyrgus antipodarum Reproduction Test
- OECD 243: Lymnaea stagnalis Reproduction Test
- Fish and other vertebrates
- OECD 203: Fish, Acute Toxicity Test
- OECD 215: Fish Juvenile Growth Study
- OECD 212: Fish, Short-term Toxicity Test on Embryo and Sac-fry Stages
- OECD 231: The Amphibian Metamorphosis Assay
- OECD 236: Fish Embryo Acute Toxicity Test
- OECD 210: Fish, Early-life Stage Toxicity Test
- OECD 229 Fish Short Term Reproduction Assay and OECD 230 21-day Fish Assay
- OECD 240 Medaka Extended One Generation Reproduction Test (MEOGRT)
- OECD 248: Xenopus Eleutheroembryonic Thyroid Assay
- OPPTS 850.1500: Fish Life Cycle Toxicity Test
- OÈCD 234 Fish sexual development test
- Aquatic plants
- Analytical Dose Verification
- Aquatic Invertebrates
- Chemistry
- Biodegradation Studies
- Analytical Chemistry Studies and Residues
- Physical-Chemical Properties Studies
- Storage Stability Studies
- OPPTS 830.6302, OPPTS 830.6303,and OPPTS 830.6304: Physical State, Colour and Odor at 20 °C and at 101.3 kPa
- EU A.1: Melting temperature/range
- EU A.2: Boiling temperature
- EU A.3: Relative density (liquids and solids)
- EU A.4: Vapour pressure
- EU A.5: Surface tension
- EU A.9: Flashpoint
- EU A.10: Flammability (solids)
- EU A.12: Flammability (contact with water)
- EU A.13: Pyrophoric properties of solids and liquids
- EU A.16: Relative self-ignition temperature for solids
- EU A.17: Oxidising properties
- OECD 114: Viscosity of Liquids
- Environmental Fate
- Transformation in Soil
- Transformation in Water
- Transformation in Manure
- Adsorption on Soil and Sewage Sludge
- Bioaccumulation and Bioconcentration
- Terrestrial Ecotoxicology
- Non-target Arthropods
- Non-target arthropod testing with the parasitic wasp (Aphidius rhopalosiphi)
- Non-target arthropod testing with the lacewing (Chrysoperla carnea)
- Non-target arthropod testing with the ladybird beetle (Coccinella septempunctata)
- Non-target arthropod testing with the predatory bug (Orius laevigatus)
- Non-target arthropod testing with the predatory mite (Typhlodromus pyri)
- Non-target arthropod testing with the rove beetle (Aleochara bilineata)
- Non-target arthropod testing with the carabid beetle (Poecilus cupreus)
- Non-target arthropod testing with the wolf spider (Pardosa spec.)
- Soil Organisms
- Honey Bees and other Pollinators
- OECD 213/214: Honey bees, Acute Oral and Acute Contact Toxicity Test
- OECD 245: Honey Bee (Apis Mellifera L.), Chronic Oral Toxicity Test (10-Day Feeding)
- OECD 237: Honey Bee Larval Toxicity Test, Single Exposure
- OECD 239: Honey Bee Larval Toxicity Test
- EPPO 170: Honey Bee Field Study – do plant protection products effect honey bee colonies?
- Oomen et al. 1992: Honey Bee Brood Feeding Study
- OECD 75: Honey Bee Brood Test under Semi-field Conditions in Tunnels
- OECD 246/247 Acute Oral and Contact Toxicity to the Bumblebee, Bombus terrestris L.
- Solitary Bee Acute Contact Toxicity Study in the Laboratory (Osmia sp.) Solitary Bee Acute Oral Toxicity Study in the Laboratory (Osmia sp.) (protocols for ringtests with solitary bees recommended by the non-Apis working group)
- SANTE/11956/2016 rev.9 Residue trials for MRL setting in honey
- Non-target plants
- OECD 208: Terrestrial Plant Test - Seedling Emergence and Seedling Growth Test
- OECD 227: Terrestrial Plant Test - Vegetative Vigour Test
- OCSPP 850.4100: Seedling Emergence and Seedling Growth
- OCSPP 850.4150: Vegetative Vigor
- EPPO PP 1/207(2): Efficacy evaluation of plant protection products, Effects on succeeding crops
- Field Studies
- Non-target Arthropods
- Ecological Modelling
- Quality Assurance
- Testing of Potential Endocrine Disruptors
- Aquatic Ecotoxicology
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Analytical Dose Verification
Every test substance sent to ibacon for testing has unique physical and chemical properties which can influence the way in which the aquatic biology studies are carried out. Substances may be poorly water soluble, prone to hydrolysis, photolysis, biological degradation, or may be extremely volatile. This can therefore falsify the results if degradation of the test substances occurs over the course of the study. Ensuring that the test plant or animal was continuously exposed to the correct concentration is therefore an essential part of any of the wide variety of biological studies conducted here at ibacon.
A dedicated team of analytical and environmental chemists works together in close collaboration with our biology team to monitor the behavior and verify the concentrations of every test substance. This allows us to decide on the most suitable test design for each test substance (e.g. semi static or flow-through test design for unstable test substances, closed vessel design for volatile test substances, etc).
The main challenge for our analytical team is to establish and adapt analytical methods which are suitable for the verification of even the lowest test concentrations down to the low µg L-1 range and below. We have a wide range of analytical instruments at our disposal that allows us to analyse almost every kind of substance. Our available instruments are:
- UPLC and HPLC with UV, DAD, or ELSD detection
- LC MS-MS
- GC-MS
- GC-FID/ECD
- IC (Ion Chromatography)
- AAS
- TOC (Total Organic Carbon)
Method Development
If a suitable analytical method cannot be provided by the sponsor, our analytical team can develop an appropriate method with the required sensitivity for the concentration range used in the biological study. Method development is carried out under non-GLP conditions. During this stage of method development an appropriate instrument and detection method is chosen (e.g. HPLC with UV detection at a specific wavelength using a specific column), along with any necessary sample preparation (e.g. dilution, filtration, or centrifugation).
After initial method development the biological pre-test will be analysed to produce information concerning the dosing of the test substance, its stability during the test and during storage of the samples, and the suitability of the analytical method. This information is vital for choosing the appropriate test design and appropriate analytical method for the main study. At this stage the development of complex sample preparation steps such as enrichment via liquid-liquid extraction or solid phase extraction, or derivatisation, may be necessary if the limit of quantification of the initial analytical method is not suitable.
Main Study and Method Verification
The final method will be validated under GLP conditions alongside the analytical measurements of the main biological study samples. The validity of the method will be determined according to the validity criteria set out in the SANCO 3029/99 guidelines. Fortified samples are prepared to check for the precision and accuracy of the method, and various blank samples are used to confirm the absence of analytical contaminants or interferences which could have entered the samples during sampling, sample preparation, or analysis. A calibration curve across the required concentration range is also prepared to confirm the linearity of the instrument response, and to be able to quantify the test substance in the biological samples. These data will be included in the final study report.
Guidelines and literature
- Chemicals Act (ChemG) of the Federal Republic of Germany, Annex 1, in the version published on 28 August 2013 (Federal Law Gazette I, p. 3498) last amended by Article 1 of the Ordinance of 20 June 2014 (Federal Law Gazette I, p. 824)
- OECD Principles of Good Laboratory Practice, adopted by Council on 26th November 1997 [C(97)186/Final], Environment Directorate, Organisation for Economic Co-operation and Development, ENV/MC/CHEM(98)17, Paris 1998
- SANCO/3029/99 rev.4 11/07/00: Residues: Guidance for generating and reporting methods of analysis in support of pre-registration data requirements for Annex II (part A; Section 4) and Annex III (part A; Section 5) of directive 91/414