- Your Product Type
- Your Study Type
- Aquatic Ecotoxicology
- Aquatic Invertebrates
- OECD 202: Daphnia sp., Acute Immobilisation Test
- OECD 211: Daphnia magna Reproduction Test
- OECD 235: Chironomus sp., Acute Immobilisation Test
- OECD 218/219: Sediment-Water Chironomid Toxicity Test Using Spiked Sediment/Spiked Water
- OECD 233: Sediment-Water Chironomid Life-Cycle Toxicity Test Using Spiked Water or Spiked Sediment
- OECD 225: Sediment-water Lumbriculus Toxicity Test Using Spiked Sediment
- OECD 242: Potamopyrgus antipodarum Reproduction Test
- OECD 243: Lymnaea stagnalis Reproduction Test
- Fish and other vertebrates
- OECD 203: Fish, Acute Toxicity Test
- OECD 215: Fish Juvenile Growth Study
- OECD 212: Fish, Short-term Toxicity Test on Embryo and Sac-fry Stages
- OECD 231: The Amphibian Metamorphosis Assay
- OECD 236: Fish Embryo Acute Toxicity Test
- OECD 210: Fish, Early-life Stage Toxicity Test
- OECD 229 Fish Short Term Reproduction Assay and OECD 230 21-day Fish Assay
- OECD 240 Medaka Extended One Generation Reproduction Test (MEOGRT)
- OECD 248: Xenopus Eleutheroembryonic Thyroid Assay
- OPPTS 850.1500: Fish Life Cycle Toxicity Test
- OÈCD 234 Fish sexual development test
- Aquatic plants
- Analytical Dose Verification
- Aquatic Invertebrates
- Chemistry
- Biodegradation Studies
- Analytical Chemistry Studies and Residues
- Physical-Chemical Properties Studies
- Storage Stability Studies
- OPPTS 830.6302, OPPTS 830.6303,and OPPTS 830.6304: Physical State, Colour and Odor at 20 °C and at 101.3 kPa
- EU A.1: Melting temperature/range
- EU A.2: Boiling temperature
- EU A.3: Relative density (liquids and solids)
- EU A.4: Vapour pressure
- EU A.5: Surface tension
- EU A.9: Flashpoint
- EU A.10: Flammability (solids)
- EU A.12: Flammability (contact with water)
- EU A.13: Pyrophoric properties of solids and liquids
- EU A.16: Relative self-ignition temperature for solids
- EU A.17: Oxidising properties
- OECD 114: Viscosity of Liquids
- Environmental Fate
- Transformation in Soil
- Transformation in Water
- Transformation in Manure
- Adsorption on Soil and Sewage Sludge
- Bioaccumulation and Bioconcentration
- Terrestrial Ecotoxicology
- Non-target Arthropods
- Non-target arthropod testing with the parasitic wasp (Aphidius rhopalosiphi)
- Non-target arthropod testing with the lacewing (Chrysoperla carnea)
- Non-target arthropod testing with the ladybird beetle (Coccinella septempunctata)
- Non-target arthropod testing with the predatory bug (Orius laevigatus)
- Non-target arthropod testing with the predatory mite (Typhlodromus pyri)
- Non-target arthropod testing with the rove beetle (Aleochara bilineata)
- Non-target arthropod testing with the carabid beetle (Poecilus cupreus)
- Non-target arthropod testing with the wolf spider (Pardosa spec.)
- Soil Organisms
- Honey Bees and other Pollinators
- OECD 213/214: Honey bees, Acute Oral and Acute Contact Toxicity Test
- OECD 245: Honey Bee (Apis Mellifera L.), Chronic Oral Toxicity Test (10-Day Feeding)
- OECD 237: Honey Bee Larval Toxicity Test, Single Exposure
- OECD 239: Honey Bee Larval Toxicity Test
- EPPO 170: Honey Bee Field Study – do plant protection products effect honey bee colonies?
- Oomen et al. 1992: Honey Bee Brood Feeding Study
- OECD 75: Honey Bee Brood Test under Semi-field Conditions in Tunnels
- OECD 246/247 Acute Oral and Contact Toxicity to the Bumblebee, Bombus terrestris L.
- Solitary Bee Acute Contact Toxicity Study in the Laboratory (Osmia sp.) Solitary Bee Acute Oral Toxicity Study in the Laboratory (Osmia sp.) (protocols for ringtests with solitary bees recommended by the non-Apis working group)
- SANTE/11956/2016 rev.9 Residue trials for MRL setting in honey
- Non-target plants
- OECD 208: Terrestrial Plant Test - Seedling Emergence and Seedling Growth Test
- OECD 227: Terrestrial Plant Test - Vegetative Vigour Test
- OCSPP 850.4100: Seedling Emergence and Seedling Growth
- OCSPP 850.4150: Vegetative Vigor
- EPPO PP 1/207(2): Efficacy evaluation of plant protection products, Effects on succeeding crops
- Field Studies
- Non-target Arthropods
- Ecological Modelling
- Quality Assurance
- Testing of Potential Endocrine Disruptors
- Aquatic Ecotoxicology
- News
- Company
- Career
- Contact
OECD 238: Sediment-free Myriophyllum spicatum toxicity test
The sediment-free higher tier study with Myriophyllum spicatum is designed to assess the toxicity of chemicals to higher plants. Test with higher aquatic plants species can be necessary for the risk assessment of plant protection products and biocides. The toxicity of different test compounds can be easily determined with the sediment-free system independent of the distribution behaviour of the compound between water and sediment. This test system has a low analytical comlexity and the obtained results can be compared with those of Lemna test results.
Study Design
Test Organisms
Myriophyllum spicatum (Eurasian water milfoil) is a submerged, rooted species which tolerates a wide range of conditions and is found in both static and flowing water bodies. M. spicatum is bred in our own sterile laboratory culture.
Course of the test
The sediment-free Myriophyllum test is a sterile test system where static or semi-static studies can be easily conducted. It is recommended for test items that e.g. degrade rapidly or which are volatile.
The objective of this 14 day-exposure test is the quantification of chemical related effects on the vegetative growth over this period. Therefore, a series of geometric test concentrations (à five replicates) and a control group (ten replicates) are assessed. Each replicate consists of one lateral branch shortened to 2.5 cm that is inserted into a test tube under aseptic conditions. At test start and test end the length and biomass of every plant will be determined.
The exposure takes place at a light intensity of 6000-9000 lux with a light:dark ratio of 16:8 hours. The temperature should be 23 ± 2 °C and the pH value should remain between 6 – 9.
Endpoints
Growth of shoot length, lateral branches and roots as well as fresh and dry weight can be easily determined with the help of the sediment-free test system. Moreover, sublethal effects such as chlorosis or necrosis are recorded. The test concentrations of at least the highest and one lower concentrations are verified at test initiation and termination.
Guidelines and literature
- OECD Guideline 238: Sediment-free Myriophyllum spicatum Toxicity Test, September 26, 2014