- Aquatic Ecotoxicology
- OECD 202: Daphnia sp., Acute Immobilisation Test
- OECD 211: Daphnia magna Reproduction Test
- OECD 235: Chironomus sp., Acute Immobilisation Test
- OECD 218/219: Sediment-Water Chironomid Toxicity Test Using Spiked Sediment/Spiked Water
- OECD 233: Sediment-Water Chironomid Life-Cycle Toxicity Test Using Spiked Water or Spiked Sediment
- OECD 225: Sediment-water Lumbriculus Toxicity Test Using Spiked Sediment
- OECD 242: Potamopyrgus antipodarum Reproduction Test
- OECD 243: Lymnea stagnalis Reproduction Test
- OECD 203: Fish, Acute Toxicity Test
- OECD 215: Fish Juvenile Growth Study
- OECD 212: Fish, Short-term Toxicity Test on Embryo and Sac-fry Stages
- OECD 231: The Amphibian Metamorphosis Assay
- OECD 236: Fish Embryo Acute Toxicity Test
- OECD 210: Fish, Early-life Stage Toxicity Test
- OECD 229 Fish Short Term Reproduction Assay and OECD 230 21-day Fish Assay
- OECD 240 Medaka Extended One Generation Reproduction Test (MEOGRT)
- OECD 241: Larval Amphibian Growth and Development Assay
- OPPTS 850.1500: Fish Life Cycle Toxicity Test
- OÈCD 234 Fish sexual development test
- Storage Stability Studies
- OPPTS 830.6302, OPPTS 830.6303,and OPPTS 830.6304: Physical State, Colour and Odor at 20 °C and at 101.3 kPa
- EU A.1: Melting temperature/range
- EU A.2: Boiling temperature
- EU A.3: Relative density (liquids and solids)
- EU A.4: Vapour pressure
- EU A.5: Surface tension
- EU A.9: Flashpoint
- EU A.10: Flammability (solids)
- EU A.12: Flammability (contact with water)
- EU A.13: Pyrophoric properties of solids and liquids
- EU A.16: Relative self-ignition temperature for solids
- EU A.17: Oxidising properties
- OECD 114: Viscosity of Liquids
- Environmental Fate
- Terrestrial Ecotoxicology
- Non-target arthropod testing with the parasitic wasp (Aphidius rhopalosiphi)
- Non-target arthropod testing with the lacewing (Chrysoperla carnea)
- Non-target arthropod testing with the ladybird beetle (Coccinella septempunctata)
- Non-target arthropod testing with the predatory bug (Orius laevigatus)
- Non-target arthropod testing with the predatory mite (Typhlodromus pyri)
- Non-target arthropod testing with the rove beetle (Aleochara bilineata)
- Non-target arthropod testing with the carabid beetle (Poecilus cupreus)
- Non-target arthropod testing with the wolf spider (Pardosa spec.)
- OECD 213/214: Honey bees, Acute Oral and Acute Contact Toxicity Test
- Honeybee: Chronic Feeding Tests
- OECD 237: Honey Bee Larval Toxicity Test, Single Exposure
- Effects on Honey Bee Brood (Apis mellifera) - Brood Feeding Test -
- OECD Draft TG: Honey Bee Larval Toxicity Test, Repeated Exposure
- OECD 75: Honey Bee Brood Test under Semi-field conditions
- EPPO 170: Honey Bee Field Study – do plant protection products effect honey bee colonies?
- Acute Oral and Contact Toxicity to the Bumblebee, Bombus terrestris L.
- Ecological Modelling
- Quality Assurance
- Testing of Potential Endocrine Disruptors
- Aquatic Ecotoxicology
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Non-target arthropod testing with the rove beetle (Aleochara bilineata)
The aim of the study is to estimate the reproduction efficiency of Aleochara bilineata under the impact of residues of plant potection products on natural soil in an extended laboratory experiment, compared to a water treated control and a reference item group. If possible the ER50 value for reproduction effects will be calculated.
The rove beetle Aleochara bilineata is recommended as one of the standard species for non-target arthropod regulatory testing for plant protection products (Candolfi et al. 2001).
Course of the test
Each treatment group will include 4 replicates with 10 female and 10 male beetles, respectively. The study will be carried out under extended laboratory conditions with natural substrate (LUFA 2.1 soil).
The larvae hatched from the eggs laid in the soil by the female beetles will parasitize the fly pupae. To assess the reproductive efficiency the number of beetles emerging from the successfully parasitized fly pupae will be counted.
Tailor-made study designs
The study can also be performed in an aged residue design. For this study type soil will be treated under field conditions and the treated soil will be transferred to the lab after aging of a determined time period. The course of the test is the same as described above.
Reproduction efficiency of exposed beetles; if possible ER50: lethal rate producing 50 % reduction in reproduction at test end.
Guidelines and Literature
- Candolfi M.P., Barrett K.L., Campbell P.J., Forster R., Grandy N., Huet M-C., Lewis G., Oomen P.A., Schmuck R. & Vogt H., 2001. Guidance document on regulatory testing and risk assessment procedures for plant protection products with non-target arthropods. From the ESCORT 2 workshop (European Standard Characteristics Of non-target arthropods Regulatory Testing), a joint BART, EOOP/CoE, OECD and IOBC Workshop organized in conjunction with SETAC-Europe and EC. SETAC Europe publisher, 2001
- Grimm et al. 2000: A test for evaluating the chronic effects of plant protection products on the rove beetle Aleochara bilineata Gyll. (Coleoptera: Staphylinidae) under laboratory and extended laboratory conditions.