- Aquatic Ecotoxicology
- OECD 202: Daphnia sp., Acute Immobilisation Test
- OECD 211: Daphnia magna Reproduction Test
- OECD 235: Chironomus sp., Acute Immobilisation Test
- OECD 218/219: Sediment-Water Chironomid Toxicity Test Using Spiked Sediment/Spiked Water
- OECD 233: Sediment-Water Chironomid Life-Cycle Toxicity Test Using Spiked Water or Spiked Sediment
- OECD 225: Sediment-water Lumbriculus Toxicity Test Using Spiked Sediment
- OECD 242: Potamopyrgus antipodarum Reproduction Test
- OECD 243: Lymnea stagnalis Reproduction Test
- OECD 203: Fish, Acute Toxicity Test
- OECD 215: Fish Juvenile Growth Study
- OECD 212: Fish, Short-term Toxicity Test on Embryo and Sac-fry Stages
- OECD 231: The Amphibian Metamorphosis Assay
- OECD 236: Fish Embryo Acute Toxicity Test
- OECD 210: Fish, Early-life Stage Toxicity Test
- OECD 229 Fish Short Term Reproduction Assay and OECD 230 21-day Fish Assay
- OECD 240 Medaka Extended One Generation Reproduction Test (MEOGRT)
- OECD 241: Larval Amphibian Growth and Development Assay
- OPPTS 850.1500: Fish Life Cycle Toxicity Test
- OÈCD 234 Fish sexual development test
- Storage Stability Studies
- OPPTS 830.6302, OPPTS 830.6303,and OPPTS 830.6304: Physical State, Colour and Odor at 20 °C and at 101.3 kPa
- EU A.1: Melting temperature/range
- EU A.2: Boiling temperature
- EU A.3: Relative density (liquids and solids)
- EU A.4: Vapour pressure
- EU A.5: Surface tension
- EU A.9: Flashpoint
- EU A.10: Flammability (solids)
- EU A.12: Flammability (contact with water)
- EU A.13: Pyrophoric properties of solids and liquids
- EU A.16: Relative self-ignition temperature for solids
- EU A.17: Oxidising properties
- OECD 114: Viscosity of Liquids
- Environmental Fate
- Terrestrial Ecotoxicology
- Non-target arthropod testing with the parasitic wasp (Aphidius rhopalosiphi)
- Non-target arthropod testing with the lacewing (Chrysoperla carnea)
- Non-target arthropod testing with the ladybird beetle (Coccinella septempunctata)
- Non-target arthropod testing with the predatory bug (Orius laevigatus)
- Non-target arthropod testing with the predatory mite (Typhlodromus pyri)
- Non-target arthropod testing with the rove beetle (Aleochara bilineata)
- Non-target arthropod testing with the carabid beetle (Poecilus cupreus)
- Non-target arthropod testing with the wolf spider (Pardosa spec.)
- OECD 213/214: Honey bees, Acute Oral and Acute Contact Toxicity Test
- OECD 245: Honey Bee (Apis Mellifera L.), Chronic Oral Toxicity Test (10-Day Feeding)
- OECD 237: Honey Bee Larval Toxicity Test, Single Exposure
- OECD 239: Honey Bee Larval Toxicity Test
- EPPO 170: Honey Bee Field Study – do plant protection products effect honey bee colonies?
- Oomen et al. 1992: Honey Bee Brood Feeding Study
- OECD 75: Honey Bee Brood Test under Semi-field Conditions in Tunnels
- OECD 246/247 Acute Oral and Contact Toxicity to the Bumblebee, Bombus terrestris L.
- Solitary Bee Acute Contact Toxicity Study in the Laboratory (Osmia sp.) Solitary Bee Acute Oral Toxicity Study in the Laboratory (Osmia sp.) (protocols for ringtests with solitary bees recommended by the non-Apis working group)
- SANTE/11956/2016 rev.9 Residue trials for MRL setting in honey
- OECD 208: Terrestrial Plant Test - Seedling Emergence and Seedling Growth Test
- OECD 227: Terrestrial Plant Test - Vegetative Vigour Test
- OCSPP 850.4100: Seedling Emergence and Seedling Growth
- OCSPP 850.4150: Vegetative Vigor
- EPPO PP 1/207(2): Efficacy evaluation of plant protection products, Effects on succeeding crops
- Ecological Modelling
- Quality Assurance
- Testing of Potential Endocrine Disruptors
- Aquatic Ecotoxicology
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EU A.9: Flashpoint
The flash point gives some indication of how easy it is to initiate the burning of this substance.
The purpose of the study is to evaluate the flash point.
It is useful to have preliminary information on the flammability of the substance before performing this test.
Course of the test
Apparatus - A flash point tester with a continuously closed cup (equivalent to the Pensky Martens method)
Test Conditions - The test will be performed at room temperature in a laboratory fume hood.
- approx. 5.5 °C/min for non-viscous liquids
- approx. 1.3 °C/min for high-viscous liquids
The test item will be placed in a test vessel which will be progressively heated until the vapour reaches a sufficiently high concentration in air to produce a flammable mixture which could be ignited by an electric arc. A flash point tester with a continuously closed cup will be used. The results of this apparatus are equivalent to the Pensky Martens method.
A preliminary test will be performed to determine the range of the flash point. The heat will be supplied at such rate that the temperature as indicated by the thermometer increases about 8.5 °C/min. An attempt will be made to ignite the vapour of the test item at each temperature that is a multiple of 5 °C, starting at room temperature or at 1 °C above the melting point (for melting points below 200 °C). No further testing will be performed if no flash point will be detected up to 400 °C.
If a flash point will be expected from the preliminary test, the main study will be performed. The heat will be supplied at such rate that the temperature as indicated by the thermometer increases about 1.3 °C/min or 5.5 °C/min. The starting temperature should be approximately 18 °C below the flash point (if applicable) found in the preliminary test, at least at 1 °C above the melting point of the test item. The flash point should be tested at each temperature that is a multiple of 1 °C. If a flash point of > 110 °C is to be expected, each temperature that is a multiple of 2 °C will be tested.
The test procedure as outlined above will be repeated after emptying and recharging until at least two successive determinations agree within the limits specified for repeatability (2 °C).
The mean flash point temperature [°C] will be reported (if applicable).
Guidelines and Literature
- Commission Regulation (EC) No 440/2008 laying down test methods pursuant to Regulation (EC) No 1907/2006 of the European Parliament and of the Council on the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) (originally published as Council Regulation (EC) No 440/2008, corrigendum according to Official Journal of the European Union (EN) dated June 3, 2008, L 143/55), Annex, Part A, A.9.: “Flash Point”, Official Journal of the European Union, L 142, Volume 51, 31 May 2008
- ASTM designation D 7094-04, Flash Point by Modified Continuosly Closed Cup (MCCCFP) Tester, November 2004