- Aquatic Ecotoxicology
- OECD 202: Daphnia sp., Acute Immobilisation Test
- OECD 211: Daphnia magna Reproduction Test
- OECD 235: Chironomus sp., Acute Immobilisation Test
- OECD 218/219: Sediment-Water Chironomid Toxicity Test Using Spiked Sediment/Spiked Water
- OECD 233: Sediment-Water Chironomid Life-Cycle Toxicity Test Using Spiked Water or Spiked Sediment
- OECD 225: Sediment-water Lumbriculus Toxicity Test Using Spiked Sediment
- OECD 242: Potamopyrgus antipodarum Reproduction Test
- OECD 243: Lymnea stagnalis Reproduction Test
- OECD 203: Fish, Acute Toxicity Test
- OECD 215: Fish Juvenile Growth Study
- OECD 212: Fish, Short-term Toxicity Test on Embryo and Sac-fry Stages
- OECD 231: The Amphibian Metamorphosis Assay
- OECD 236: Fish Embryo Acute Toxicity Test
- OECD 210: Fish, Early-life Stage Toxicity Test
- OECD 229 Fish Short Term Reproduction Assay and OECD 230 21-day Fish Assay
- OECD 240 Medaka Extended One Generation Reproduction Test (MEOGRT)
- OECD 241: Larval Amphibian Growth and Development Assay
- OPPTS 850.1500: Fish Life Cycle Toxicity Test
- OÈCD 234 Fish sexual development test
- Storage Stability Studies
- OPPTS 830.6302, OPPTS 830.6303,and OPPTS 830.6304: Physical State, Colour and Odor at 20 °C and at 101.3 kPa
- EU A.1: Melting temperature/range
- EU A.2: Boiling temperature
- EU A.3: Relative density (liquids and solids)
- EU A.4: Vapour pressure
- EU A.5: Surface tension
- EU A.9: Flashpoint
- EU A.10: Flammability (solids)
- EU A.12: Flammability (contact with water)
- EU A.13: Pyrophoric properties of solids and liquids
- EU A.16: Relative self-ignition temperature for solids
- EU A.17: Oxidising properties
- OECD 114: Viscosity of Liquids
- Environmental Fate
- Terrestrial Ecotoxicology
- Non-target arthropod testing with the parasitic wasp (Aphidius rhopalosiphi)
- Non-target arthropod testing with the lacewing (Chrysoperla carnea)
- Non-target arthropod testing with the ladybird beetle (Coccinella septempunctata)
- Non-target arthropod testing with the predatory bug (Orius laevigatus)
- Non-target arthropod testing with the predatory mite (Typhlodromus pyri)
- Non-target arthropod testing with the rove beetle (Aleochara bilineata)
- Non-target arthropod testing with the carabid beetle (Poecilus cupreus)
- Non-target arthropod testing with the wolf spider (Pardosa spec.)
- OECD 213/214: Honey bees, Acute Oral and Acute Contact Toxicity Test
- Honeybee: Chronic Feeding Tests
- OECD 237: Honey Bee Larval Toxicity Test, Single Exposure
- Effects on Honey Bee Brood (Apis mellifera) - Brood Feeding Test -
- OECD Draft TG: Honey Bee Larval Toxicity Test, Repeated Exposure
- OECD 75: Honey Bee Brood Test under Semi-field conditions
- EPPO 170: Honey Bee Field Study – do plant protection products effect honey bee colonies?
- Acute Oral and Contact Toxicity to the Bumblebee, Bombus terrestris L.
- Ecological Modelling
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- Testing of Potential Endocrine Disruptors
- Aquatic Ecotoxicology
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EU A.2: Boiling temperature
The boiling point of a substance is an environmentally relevant physical chemical property because it is important for identification purposes and is one factor influencing the states in which the substance will exist in the environment.
The purpose of this study is to evaluate the boiling point by the capillary method using an electronic controlled heating system.
Course of the test
Apparatus - Büchi B 545
The boiling point will be determined using a Büchi B 545 boiling point device. The temperature range of the device is from room temperature up to 400 °C. The boiling temperature is that temperature at which, on momentary cooling, the formation of bubbles stops and the liquid suddenly rises in the capillary.
For the determination of the boiling point, the capillary method according to Siwoloboff will be used. A small amount of the test item will be heated in the electronic controlled sample block and the temperature of around the sample will be displayed. The sample will be observed visually while raising the temperature. A phase transformation from liquid to vapour usually is deduced by the formation of steam bubbles leaving the inserted boiling capillary.
The boiling behaviour of the test item will be estimated in a preliminary test. Therefore, a sample of the test item in a glass tube will be heated from room temperature up to about 400 °C with a rate of approximately 10 °C/min unless a phase transition or reaction of the test item will be observed.
If in the temperature range investigated in the preliminary test, a phase transition of the test item is observed, the experiment will be repeated with a new test item aliquot in the immediate vicinity of the temperature of the phase transition observed. For the actual determination of the boiling point, the temperature rise will be adjusted to a rate less than of 1 °C/min at about 15 °C before and after the predetermined phase transition temperature.
The boiling temperature [°C and K] at 20 °C will be reported.
In case of boiling point cannot be determined, because of decomposition or sublimation, the temperature at which the decomposition or sublimation is suspected - will be reported.
Guidelines and literature
- Commission Regulation (EC) No 440/2008 laying down test methods pursuant to Regulation (EC) No 1907/2006 of the European Parliament and of the Council on the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) (originally published as Council Regulation (EC) No 440/2008, corrigendum according to Official Journal of the European Union (EN) dated June 3, 2008, L 143/55), Annex, Part A, A.2.: “Boiling Temperature”, Official Journal of the European Union, L 142, Volume 51, 31 May 2008
- Organisation for Economic Co-operation and Development (OECD), Guidelines for Testing of Chemicals, Guideline No. 103, “Boiling Point”; adopted July 27, 1995