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- OECD 233: Sediment-Water Chironomid Life-Cycle Toxicity Test Using Spiked Water or Spiked Sediment
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- OECD 213/214: Honey bees, Acute Oral and Acute Contact Toxicity Test
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OECD 225: Sediment-water Lumbriculus Toxicity Test Using Spiked Sediment
Tests with aquatic oligochaetes such as L. variegatus are used to assess the prolonged exposure of endobenthic living animals to sediment-associated chemicals.
The method is described for static test conditions with spiked sediment simulating a sediment contaminated with the test compound. Substances that need to be tested towards sediment-dwelling organisms may be exposed via several routes e.g. contact with the test item, ingestion of contaminated sediment particles, porewater and overlying water. Moreover, test substances can persist for a long time in the compartment and therefore tests with a longer exposure period are necessary.
Test organisms
Among sediment-ingesters, oligochaetes play an important role in the sediments of aquatic systems. Lumbriculus variegatus is a widespread freshwater oligochaete being tolerant towards a wide range of sediment types and it is widely used for sediment toxicity and bioaccumulation test. The worms can be easily cultivated and are obtained from ibacon´s in-house culture.
Course of the test
Worms of a similar physiological state and size are exposed to a geometric series of test concentrations for 28 days.
Endpoints
- effects on reproduction and biomass of the oligochaetes are assessed by determination of the total number of surviving worms and the biomass at test end.
- Preferred endpoint is the calculation of the ECx.
Guidelines and literature
- OECD Guideline 225: Sediment-Water Lumbriuclus Toxicity Test Using Spiked Sediment.