- Aquatic Ecotoxicology
- OECD 202: Daphnia sp., Acute Immobilisation Test
- OECD 211: Daphnia magna Reproduction Test
- OECD 235: Chironomus sp., Acute Immobilisation Test
- OECD 218/219: Sediment-Water Chironomid Toxicity Test Using Spiked Sediment/Spiked Water
- OECD 233: Sediment-Water Chironomid Life-Cycle Toxicity Test Using Spiked Water or Spiked Sediment
- OECD 225: Sediment-water Lumbriculus Toxicity Test Using Spiked Sediment
- OECD 242: Potamopyrgus antipodarum Reproduction Test
- OECD 243: Lymnea stagnalis Reproduction Test
- OECD 203: Fish, Acute Toxicity Test
- OECD 215: Fish Juvenile Growth Study
- OECD 212: Fish, Short-term Toxicity Test on Embryo and Sac-fry Stages
- OECD 231: The Amphibian Metamorphosis Assay
- OECD 236: Fish Embryo Acute Toxicity Test
- OECD 210: Fish, Early-life Stage Toxicity Test
- OECD 229 Fish Short Term Reproduction Assay and OECD 230 21-day Fish Assay
- OECD 240 Medaka Extended One Generation Reproduction Test (MEOGRT)
- OECD 241: Larval Amphibian Growth and Development Assay
- OPPTS 850.1500: Fish Life Cycle Toxicity Test
- OÈCD 234 Fish sexual development test
- Storage Stability Studies
- OPPTS 830.6302, OPPTS 830.6303,and OPPTS 830.6304: Physical State, Colour and Odor at 20 °C and at 101.3 kPa
- EU A.1: Melting temperature/range
- EU A.2: Boiling temperature
- EU A.3: Relative density (liquids and solids)
- EU A.4: Vapour pressure
- EU A.5: Surface tension
- EU A.9: Flashpoint
- EU A.10: Flammability (solids)
- EU A.12: Flammability (contact with water)
- EU A.13: Pyrophoric properties of solids and liquids
- EU A.16: Relative self-ignition temperature for solids
- EU A.17: Oxidising properties
- OECD 114: Viscosity of Liquids
- Environmental Fate
- Terrestrial Ecotoxicology
- Non-target arthropod testing with the parasitic wasp (Aphidius rhopalosiphi)
- Non-target arthropod testing with the lacewing (Chrysoperla carnea)
- Non-target arthropod testing with the ladybird beetle (Coccinella septempunctata)
- Non-target arthropod testing with the predatory bug (Orius laevigatus)
- Non-target arthropod testing with the predatory mite (Typhlodromus pyri)
- Non-target arthropod testing with the rove beetle (Aleochara bilineata)
- Non-target arthropod testing with the carabid beetle (Poecilus cupreus)
- Non-target arthropod testing with the wolf spider (Pardosa spec.)
- OECD 213/214: Honey bees, Acute Oral and Acute Contact Toxicity Test
- Honeybee: Chronic Feeding Tests
- OECD 237: Honey Bee Larval Toxicity Test, Single Exposure
- Effects on Honey Bee Brood (Apis mellifera) - Brood Feeding Test -
- OECD Draft TG: Honey Bee Larval Toxicity Test, Repeated Exposure
- OECD 75: Honey Bee Brood Test under Semi-field conditions
- EPPO 170: Honey Bee Field Study – do plant protection products effect honey bee colonies?
- Acute Oral and Contact Toxicity to the Bumblebee, Bombus terrestris L.
- Ecological Modelling
- Quality Assurance
- Testing of Potential Endocrine Disruptors
- Aquatic Ecotoxicology
- Who we are
- Company history
- Our Certificates
EU A.3: Relative density (liquids and solids)
The density of a substance is environmentally relevant because it is helpful in estimating the distribution of the substance within and between water, soil and air.
The purpose of the study is to determine the relative density at a specific temperature with a pycnometer in case of liquid test item or by means of a gas comparison pycnometer for the solid test item.
Course of the test
Apparatus - Glass-pycnometer
Test Condition - 20 °C ± 1.0 °C
The density will be calculated from the difference in weight between the full and empty pycnometer and its known volume. A calibrated pycnometer (with stopper) will be used. It will be immersed up to its neck in a constant temperature water bath. After withdrawing the flask will be wiped with a minimum of handling. The pycnometer will be weighed after thermal equilibrium with the surrounding will have been established. Then the pycnometer will be filled with the test item. Care will be taken to avoid air bubbles when filling. The pycnometer will be immersed to the top of its neck in a water bath maintained at constant temperature and will be left in the bath for at least 20 min. The pycnometer will be removed from the bath. Afterwards the stopper will be immersed. The liquid on the neck will be removed with a piece of paper. Finally the top will be dried taking care not to withdraw liquid from the capillary and it will be weighed.
The test procedure will be repeated at least twice using independent test item samples.
Apparatus - Gas comparison pycnometer
Test Condition - 20 °C ± 1.0 °C
The sample cell will be weighed, filled with the test item and placed in the Multi-Pycnometer.
Afterwards the test will be performed. The procedure will be repeated until reproducible results are obtained. The sample cell containing test item will be reweighed to determine the mass of the tested test item.
At least two measurements performed.
The density [g/cm³] and the relative density at 20 °C will be reported.
Guidelines and Literature
- Commission Regulation (EC) No 440/2008 laying down test methods pursuant to Regulation (EC) No 1907/2006 of the European Parliament and of the Council on the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) (originally published as Council Regulation (EC) No 440/2008, corrigendum according to Official Journal of the European Union (EN) dated June 3, 2008, L 143/55), Annex, Part A, A.3.: “Relative Density”, Official Journal of the European Union, L 142, Volume 51, 31 May 2008
- Organisation for Economic Co-operation and Development (OECD), OECD Guidelines for Testing of Chemicals, Guideline No. 109, “Density of Liquids and Solids”, adopted October 02, 2012