- Aquatic Ecotoxicology
- OECD 202: Daphnia sp., Acute Immobilisation Test
- OECD 211: Daphnia magna Reproduction Test
- OECD 235: Chironomus sp., Acute Immobilisation Test
- OECD 218/219: Sediment-Water Chironomid Toxicity Test Using Spiked Sediment/Spiked Water
- OECD 233: Sediment-Water Chironomid Life-Cycle Toxicity Test Using Spiked Water or Spiked Sediment
- OECD 225: Sediment-water Lumbriculus Toxicity Test Using Spiked Sediment
- OECD 242: Potamopyrgus antipodarum Reproduction Test
- OECD 243: Lymnea stagnalis Reproduction Test
- OECD 203: Fish, Acute Toxicity Test
- OECD 215: Fish Juvenile Growth Study
- OECD 212: Fish, Short-term Toxicity Test on Embryo and Sac-fry Stages
- OECD 231: The Amphibian Metamorphosis Assay
- OECD 236: Fish Embryo Acute Toxicity Test
- OECD 210: Fish, Early-life Stage Toxicity Test
- OECD 229 Fish Short Term Reproduction Assay and OECD 230 21-day Fish Assay
- OECD 240 Medaka Extended One Generation Reproduction Test (MEOGRT)
- OECD 241: Larval Amphibian Growth and Development Assay
- OPPTS 850.1500: Fish Life Cycle Toxicity Test
- OÈCD 234 Fish sexual development test
- Storage Stability Studies
- OPPTS 830.6302, OPPTS 830.6303,and OPPTS 830.6304: Physical State, Colour and Odor at 20 °C and at 101.3 kPa
- EU A.1: Melting temperature/range
- EU A.2: Boiling temperature
- EU A.3: Relative density (liquids and solids)
- EU A.4: Vapour pressure
- EU A.5: Surface tension
- EU A.9: Flashpoint
- EU A.10: Flammability (solids)
- EU A.12: Flammability (contact with water)
- EU A.13: Pyrophoric properties of solids and liquids
- EU A.16: Relative self-ignition temperature for solids
- EU A.17: Oxidising properties
- OECD 114: Viscosity of Liquids
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- Terrestrial Ecotoxicology
- Non-target arthropod testing with the parasitic wasp (Aphidius rhopalosiphi)
- Non-target arthropod testing with the lacewing (Chrysoperla carnea)
- Non-target arthropod testing with the ladybird beetle (Coccinella septempunctata)
- Non-target arthropod testing with the predatory bug (Orius laevigatus)
- Non-target arthropod testing with the predatory mite (Typhlodromus pyri)
- Non-target arthropod testing with the rove beetle (Aleochara bilineata)
- Non-target arthropod testing with the carabid beetle (Poecilus cupreus)
- Non-target arthropod testing with the wolf spider (Pardosa spec.)
- OECD 213/214: Honey bees, Acute Oral and Acute Contact Toxicity Test
- Honeybee: Chronic Feeding Tests
- OECD 237: Honey Bee Larval Toxicity Test, Single Exposure
- Effects on Honey Bee Brood (Apis mellifera) - Brood Feeding Test -
- OECD Draft TG: Honey Bee Larval Toxicity Test, Repeated Exposure
- OECD 75: Honey Bee Brood Test under Semi-field conditions
- EPPO 170: Honey Bee Field Study – do plant protection products effect honey bee colonies?
- Acute Oral and Contact Toxicity to the Bumblebee, Bombus terrestris L.
- Ecological Modelling
- Quality Assurance
- Testing of Potential Endocrine Disruptors
- Aquatic Ecotoxicology
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OECD 111: Hydrolysis as a Function of pH
The purpose of the test is to determine the rate of hydrolysis of a chemical in water at different environmentally relevant pH-values by quantifying the test substance concentration after different incubation periods and at different temperatures. The rate of hydrolysis of the test sustance as a function of pH and the transformation product pattern will be investigated.
Non-labelled or 14C-labelled test substances can be used to measure the rate of hydrolysis. Incubation in sterilised (buffered) aqueous solution will be accomplished at different pH values (4-9) and at different temperatures in the dark. The test will be conducted using multiple flask design. Stoppered glass flasks will be used for carrying out the tests. All glassware and water will be sterilised before use. A temperature controlled water bath will be used for the incubation of the samples.
Course of the test
One test substance concentration will be used for the test. In a preliminary test the hydrolytic behaviour of the substance will be estimated. The test substance will be incubated at 50°C at three different pH values. If less than 10% hydrolysis will be observed after 5 days of incubation no additional testing is required. At those pH values were the test substance will be found to be unstable, the test will be continued and a main test is carried out. Besides 50°C, at least two further temperatures in the range of <25°C to 70°C will be tested. Each test will be executed until 90% hydrolysis of the test item will be observed or for 30 days whichever will come first. Apart from samples taken directly after application, at least 6 sampling time points will be included. Time intervals will be chosen in such a way that the pattern of decline of the test substance can be established. In case of the use of radiolabelled test substances mass balancing and transformation product pattern will be determined for the test substance.
Analysis of the test substance and transformation products
In case of the 14C-labelled test substance the analytical methods will base on LSC and HPLC coupled with UV and radio detection. LC-MS/MS can be carried out as additional analytical method. Non-labelled test substances will be determined using HPLC coupled with UV detection or LC-MS/MS method. The analytical method and sample preparation will be established to allow a limit of quantification of ≤ 5% of the applied radioactivity.
Special test setup
- The test can also be performed at pH values other than 4, 7 and 9, if required.
- The test can also be performed at lower temperatures (e.g. 12°C).
- Calculation of DT50 values
- Hydrolysis rate constant as a function of temperature, if possible
- Transformation product pattern including characterisation/ identification of major products
Guidelines and Literature
- OECD Guideline for Testing of Chemicals, No. 111, "Hydrolysis as a function of pH", adopted April 13, 2004.