- Aquatic Ecotoxicology
- OECD 202: Daphnia sp., Acute Immobilisation Test
- OECD 211: Daphnia magna Reproduction Test
- OECD 235: Chironomus sp., Acute Immobilisation Test
- OECD 218/219: Sediment-Water Chironomid Toxicity Test Using Spiked Sediment/Spiked Water
- OECD 233: Sediment-Water Chironomid Life-Cycle Toxicity Test Using Spiked Water or Spiked Sediment
- OECD 225: Sediment-water Lumbriculus Toxicity Test Using Spiked Sediment
- OECD 242: Potamopyrgus antipodarum Reproduction Test
- OECD 243: Lymnea stagnalis Reproduction Test
- OECD 203: Fish, Acute Toxicity Test
- OECD 215: Fish Juvenile Growth Study
- OECD 212: Fish, Short-term Toxicity Test on Embryo and Sac-fry Stages
- OECD 231: The Amphibian Metamorphosis Assay
- OECD 236: Fish Embryo Acute Toxicity Test
- OECD 210: Fish, Early-life Stage Toxicity Test
- OECD 229 Fish Short Term Reproduction Assay and OECD 230 21-day Fish Assay
- OECD 240 Medaka Extended One Generation Reproduction Test (MEOGRT)
- OECD 241: Larval Amphibian Growth and Development Assay
- OPPTS 850.1500: Fish Life Cycle Toxicity Test
- OÈCD 234 Fish sexual development test
- Storage Stability Studies
- OPPTS 830.6302, OPPTS 830.6303,and OPPTS 830.6304: Physical State, Colour and Odor at 20 °C and at 101.3 kPa
- EU A.1: Melting temperature/range
- EU A.2: Boiling temperature
- EU A.3: Relative density (liquids and solids)
- EU A.4: Vapour pressure
- EU A.5: Surface tension
- EU A.9: Flashpoint
- EU A.10: Flammability (solids)
- EU A.12: Flammability (contact with water)
- EU A.13: Pyrophoric properties of solids and liquids
- EU A.16: Relative self-ignition temperature for solids
- EU A.17: Oxidising properties
- OECD 114: Viscosity of Liquids
- Environmental Fate
- Terrestrial Ecotoxicology
- Non-target arthropod testing with the parasitic wasp (Aphidius rhopalosiphi)
- Non-target arthropod testing with the lacewing (Chrysoperla carnea)
- Non-target arthropod testing with the ladybird beetle (Coccinella septempunctata)
- Non-target arthropod testing with the predatory bug (Orius laevigatus)
- Non-target arthropod testing with the predatory mite (Typhlodromus pyri)
- Non-target arthropod testing with the rove beetle (Aleochara bilineata)
- Non-target arthropod testing with the carabid beetle (Poecilus cupreus)
- Non-target arthropod testing with the wolf spider (Pardosa spec.)
- OECD 213/214: Honey bees, Acute Oral and Acute Contact Toxicity Test
- Honeybee: Chronic Feeding Tests
- OECD 237: Honey Bee Larval Toxicity Test, Single Exposure
- Effects on Honey Bee Brood (Apis mellifera) - Brood Feeding Test -
- OECD Draft TG: Honey Bee Larval Toxicity Test, Repeated Exposure
- OECD 75: Honey Bee Brood Test under Semi-field conditions
- EPPO 170: Honey Bee Field Study – do plant protection products effect honey bee colonies?
- Acute Oral and Contact Toxicity to the Bumblebee, Bombus terrestris L.
- Ecological Modelling
- Quality Assurance
- Testing of Potential Endocrine Disruptors
- Aquatic Ecotoxicology
- Who we are
- Company history
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OECD 301/310: Ready Biodegradability Tests
Biodegradation is one of the most important factors in assessing the environmental fate of chemicals. Substances coming into the aquatic environment via waste water get in contact with the sewage treatment microorganisms, which is therefore the basis for the assessment of aquatic biodegradation. Standard OECD 301 tests performed at ibacon are the 301D: Closed Bottle Test, the 301F: Manometric Respirometry Test and the 310: CO2 in Sealed Vessels (Headspace) Test.
Selection of adequate test
Depending on the test item specific properties such as water solubility, different tests are applicable:
Non- / Slight Volatile
|Oxygen concentration (Oxygen Electrode)|
|OECD 301F||Manometric Respirometry Test||
Not Water Soluble / Soluble
Non-Volatile / Volatile
|CO2 pressure decrease (BSB-Sensomat flasks)|
|OECD 310||CO2-Headspace Test||
Non-Volatile / Volatile
by TOC Analyser
General test design
Activated sludge is taken from the aeration tank of a domestic waste water treatment plant. After a conditioning procedure defined suspensions of the activated sludge microorganisms are introduced in the test vessels.
Beside the test item treatments a series of controls can be performed: control, reference item and toxicity control. The control contains only activated sludge. As a highly degradable reference item, e.g. Sodim Benzoate is used to check the activity of the sludge microorganisms. The toxicity control contains the test item and the reference item to detect inhibitory effects of the test item.
For test items containing nitrogen, the nitrification can be measured by Continuous Flow Analysis according to Annex V. For poorly souluble test items an application onto silica gel or an addition of silicon oil is possible. All tests are carried out in controlled environment rooms with constant temperature in the dark with a standard test duration of 28 days.
OECD 301D: Closed Bottle Test
A sufficient number of Karlsruher flasks are bubble-free filled with test solutions and tightly closed by glass stoppers. The biodegradation is followed by the oxygen uptake of the microorganisms during exposure. The oxygen concentrations are measured electrochemically at eight sampling dates in the airtight system with an O2-electrode under constant stirring. The percentage of degradation is determined by correction for the blank and under consideration of the theoretical oxygen demand of the test item. An application of this test is only possible for water soluble test items due to the fact that a stock solution of the test item is prepared and the test flasks are not continously stirred during the incubation time.
OECD 301F: Manometric Respirometry Test
Biodegradation is followed by the oxygen uptake of the micro-organisms. A manometric system is used to measure the pressure decrease in the test vessels. The bottles are remaining closed during the whole experimental time and are stirred continuously.
The percentage of degradation is determined by correction for the blank and under consideration of the theoretical oxygen demand of the test item. An application of this test is only possible for non toxic substances because relatively high amounts of the test substance are used.
OECD 310: CO2 in Sealed Vessels (Headspace) Test
The test vessels are filled with test solutions and are sealed with gas-tight septum caps. At eight sampling dates, the biodegradation is determined by following the CO2 evolution of the test item in the incubation flasks during exposure. The produced CO2 is converted to carbonate and subjected to TIC (total inorganic carbon) analysis. The percentage of degradation is determined by correction for the blank and under consideration of thecarbon content of the test item.
Test items that reach 60 % biodegradation within the 10-day window (the 10 days after passing 10%biodegradation of the test item), can be clasified as ready biodegradable. Test items that reach 60% after the 10-day window are clasified as ready biodegradable, but failing 10-day window.
Guidelines and Literature
- OECD Guideline for Testing of Chemicals No. 301 D: "Ready Biodegradability: Closed Bottle Test", adopted July 17, 1992
- OECD Guideline for Testing of Chemicals No. 301 F: "Ready Biodegradability: Manometric Respirometry Test", adopted July 17, 1992