- Aquatic Ecotoxicology
- OECD 202: Daphnia sp., Acute Immobilisation Test
- OECD 211: Daphnia magna Reproduction Test
- OECD 235: Chironomus sp., Acute Immobilisation Test
- OECD 218/219: Sediment-Water Chironomid Toxicity Test Using Spiked Sediment/Spiked Water
- OECD 233: Sediment-Water Chironomid Life-Cycle Toxicity Test Using Spiked Water or Spiked Sediment
- OECD 225: Sediment-water Lumbriculus Toxicity Test Using Spiked Sediment
- OECD 242: Potamopyrgus antipodarum Reproduction Test
- OECD 243: Lymnea stagnalis Reproduction Test
- OECD 203: Fish, Acute Toxicity Test
- OECD 215: Fish Juvenile Growth Study
- OECD 212: Fish, Short-term Toxicity Test on Embryo and Sac-fry Stages
- OECD 231: The Amphibian Metamorphosis Assay
- OECD 236: Fish Embryo Acute Toxicity Test
- OECD 210: Fish, Early-life Stage Toxicity Test
- OECD 229 Fish Short Term Reproduction Assay and OECD 230 21-day Fish Assay
- OECD 240 Medaka Extended One Generation Reproduction Test (MEOGRT)
- OECD 241: Larval Amphibian Growth and Development Assay
- OPPTS 850.1500: Fish Life Cycle Toxicity Test
- OÈCD 234 Fish sexual development test
- Storage Stability Studies
- OPPTS 830.6302, OPPTS 830.6303,and OPPTS 830.6304: Physical State, Colour and Odor at 20 °C and at 101.3 kPa
- EU A.1: Melting temperature/range
- EU A.2: Boiling temperature
- EU A.3: Relative density (liquids and solids)
- EU A.4: Vapour pressure
- EU A.5: Surface tension
- EU A.9: Flashpoint
- EU A.10: Flammability (solids)
- EU A.12: Flammability (contact with water)
- EU A.13: Pyrophoric properties of solids and liquids
- EU A.16: Relative self-ignition temperature for solids
- EU A.17: Oxidising properties
- OECD 114: Viscosity of Liquids
- Environmental Fate
- Terrestrial Ecotoxicology
- Non-target arthropod testing with the parasitic wasp (Aphidius rhopalosiphi)
- Non-target arthropod testing with the lacewing (Chrysoperla carnea)
- Non-target arthropod testing with the ladybird beetle (Coccinella septempunctata)
- Non-target arthropod testing with the predatory bug (Orius laevigatus)
- Non-target arthropod testing with the predatory mite (Typhlodromus pyri)
- Non-target arthropod testing with the rove beetle (Aleochara bilineata)
- Non-target arthropod testing with the carabid beetle (Poecilus cupreus)
- Non-target arthropod testing with the wolf spider (Pardosa spec.)
- OECD 213/214: Honey bees, Acute Oral and Acute Contact Toxicity Test
- OECD 245: Honey Bee (Apis Mellifera L.), Chronic Oral Toxicity Test (10-Day Feeding)
- OECD 237: Honey Bee Larval Toxicity Test, Single Exposure
- OECD 239: Honey Bee Larval Toxicity Test
- EPPO 170: Honey Bee Field Study – do plant protection products effect honey bee colonies?
- Oomen et al. 1992: Honey Bee Brood Feeding Study
- OECD 75: Honey Bee Brood Test under Semi-field Conditions in Tunnels
- OECD 246/247 Acute Oral and Contact Toxicity to the Bumblebee, Bombus terrestris L.
- Solitary Bee Acute Contact Toxicity Study in the Laboratory (Osmia sp.) Solitary Bee Acute Oral Toxicity Study in the Laboratory (Osmia sp.) (protocols for ringtests with solitary bees recommended by the non-Apis working group)
- SANTE/11956/2016 rev.9 Residue trials for MRL setting in honey
- OECD 208: Terrestrial Plant Test - Seedling Emergence and Seedling Growth Test
- OECD 227: Terrestrial Plant Test - Vegetative Vigour Test
- OCSPP 850.4100: Seedling Emergence and Seedling Growth
- OCSPP 850.4150: Vegetative Vigor
- EPPO PP 1/207(2): Efficacy evaluation of plant protection products, Effects on succeeding crops
- Ecological Modelling
- Quality Assurance
- Testing of Potential Endocrine Disruptors
- Aquatic Ecotoxicology
- Who we are
- Company history
- Our Certificates
OECD 201: Freshwater Alga and Cyanobacteria, Growth Inhibition Test
Algae are primary producers in freshwater and marine ecosystems. They provide the basis of the aquatic food chain. Herbivorous organisms depend directly on algae as nutrition. But also the consumers are indirectly concerned via the trophic cascade. Therefore, possible toxic effects on the growth of algae have to be assessed for plant protection products, biocides, industrial chemicals and medicinal products for human and veterinary use that may get into aquatic environment. Algae are very sensitive to xenobiotics and standardised tests systems are established since many years.
We offer tests with different algae species, which are supplied by official breeders and cultivated in the IBACON laboratory under standardised conditions.
Pseudokirchneriella subcapitata (recently renamed as Raphidocelis subcapitata, formerly known as Selenastrum capricornutum)
Course of the test
The test is performed with three replicates per test item group and six replicates in the control. The test item is mixed into the test water homogenously. Afterwards, algae are added to achieve a species dependent initial algal cell number per water volume. Cell densities are determined during the test and at the end of the test by examining samples from the flasks by spectrophotometry or microscopy. Test substance concentrations are analysed at test start and test end to verify dosage of the test item. A toxic reference, tested twice per year, proofs the sensitivity of the test system.
Closed Vessel Studies
Test items with high vapour pressure are tested in closed vessels to avoid evaporation of the test item. Furthermore adaptions of the test media are necessary to compensate the missing air exchange.
The recovery potential of algae can be monitored with recovery studies. After an acute algal test, an aliquot of some samples is introduced in pure test medium and the samples are exposed for some further days. Growth of the algae is compared to the growth of a control.
Species Sensitivity Destribution
In order to determine a species sensitivity distribution (SSD), additional algae species can be tested on request e.g. chlorella vulgaris or tetraedron caudatum.
Cell densities of the algae or Cyanobacteria are determined after 24, 48, 72 and, if required, after 96 hours. Growth is measured as the increase of cell density over the test period. EC50 (EC20, EC10), NOEC and LOEC are calculated for growth rate, yield and/or biomass. Additionally, algal shape is observed microscopically at the end of the test.
Guidelines and Literature
- OECD Guideline for Testing of Chemicals, No. 201: "Freshwater Alga and Cyanobacteria, Growth Inhibition"
- Commission Regulation (EC) No 761/2009 amending Regulation (EC) No 440/2008, Annex, Part C, C.3.: "Algal Inhibition Test", p. 36-56, Official Journal of the European Union (EN)
- EPA Ecological Effects Test Guidelines OPPTS 850.5400 Algal Toxicity, Tiers I and II