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- OECD 202: Daphnia sp., Acute Immobilisation Test
- OECD 211: Daphnia magna Reproduction Test
- OECD 235: Chironomus sp., Acute Immobilisation Test
- OECD 218/219: Sediment-Water Chironomid Toxicity Test Using Spiked Sediment/Spiked Water
- OECD 233: Sediment-Water Chironomid Life-Cycle Toxicity Test Using Spiked Water or Spiked Sediment
- OECD 225: Sediment-water Lumbriculus Toxicity Test Using Spiked Sediment
- OECD 242: Potamopyrgus antipodarum Reproduction Test
- OECD 243: Lymnaea stagnalis Reproduction Test
- Fish and other vertebrates
- OECD 203: Fish, Acute Toxicity Test
- OECD 215: Fish Juvenile Growth Study
- OECD 212: Fish, Short-term Toxicity Test on Embryo and Sac-fry Stages
- OECD 231: The Amphibian Metamorphosis Assay
- OECD 236: Fish Embryo Acute Toxicity Test
- OECD 210: Fish, Early-life Stage Toxicity Test
- OECD 229 Fish Short Term Reproduction Assay and OECD 230 21-day Fish Assay
- OECD 240 Medaka Extended One Generation Reproduction Test (MEOGRT)
- OECD 248: Xenopus Eleutheroembryonic Thyroid Assay
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- OPPTS 830.6302, OPPTS 830.6303,and OPPTS 830.6304: Physical State, Colour and Odor at 20 °C and at 101.3 kPa
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- Non-target Arthropods
- Non-target arthropod testing with the parasitic wasp (Aphidius rhopalosiphi)
- Non-target arthropod testing with the lacewing (Chrysoperla carnea)
- Non-target arthropod testing with the ladybird beetle (Coccinella septempunctata)
- Non-target arthropod testing with the predatory bug (Orius laevigatus)
- Non-target arthropod testing with the predatory mite (Typhlodromus pyri)
- Non-target arthropod testing with the rove beetle (Aleochara bilineata)
- Non-target arthropod testing with the carabid beetle (Poecilus cupreus)
- Non-target arthropod testing with the wolf spider (Pardosa spec.)
- Soil Organisms
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- OECD 213/214: Honey bees, Acute Oral and Acute Contact Toxicity Test
- OECD 245: Honey Bee (Apis Mellifera L.), Chronic Oral Toxicity Test (10-Day Feeding)
- OECD 237: Honey Bee Larval Toxicity Test, Single Exposure
- OECD 239: Honey Bee Larval Toxicity Test
- EPPO 170: Honey Bee Field Study – do plant protection products effect honey bee colonies?
- Oomen et al. 1992: Honey Bee Brood Feeding Study
- OECD 75: Honey Bee Brood Test under Semi-field Conditions in Tunnels
- OECD 246/247 Acute Oral and Contact Toxicity to the Bumblebee, Bombus terrestris L.
- Solitary Bee Acute Contact Toxicity Study in the Laboratory (Osmia sp.) Solitary Bee Acute Oral Toxicity Study in the Laboratory (Osmia sp.) (protocols for ringtests with solitary bees recommended by the non-Apis working group)
- SANTE/11956/2016 rev.9 Residue trials for MRL setting in honey
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- OECD 208: Terrestrial Plant Test - Seedling Emergence and Seedling Growth Test
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Non-target arthropod testing with the wolf spider (Pardosa spec.)
The aim of the study is to estimate the acute toxicity of Pardosa spec. under the impact of residues of plant potection products on natural soil in an extended laboratory experiment, compared to a water treated control and a reference item group. Additionally, an assessment for significant sublethal effects will be made (behavioural abnormalities and food consumption). If possible the EC50 value for mortality will be calculated.
Study Design
Test organisms
The wolf spider Pardosa spec. is recommended as one of the standard species for non-target arthropod regulatory testing for plant protection products (Candolfi et al. 2001).
Course of the test
The purpose of this study is to determine the toxicity of plant protection products on the wolf spider Pardosa spec. under extended laboratory conditions and to investigate sublethal effects on behaviour and food consumption of the survivors, compared to a water treated control and to a reference item. The bioassay will be carried out under extended laboratory conditions with natural substrate (LUFA 2.1 soil). The exposure time of the spiders to the treated soil is 14 days.
Tailor-made study designs
The study can also be performed in an aged residue design. For this study type soil will be treated under field conditions and the treated soil will be transferred to the lab after aging of a determined time period. The course of the test is the same as described above.
Endpoints
Mortality, behavioural abnormalities and food consumption of exposed spiders; if possible EC50: lethal rate producing 50 % mortality at test end (14 days).
Guidelines and Literature
- Candolfi M.P., Barrett K.L., Campbell P.J., Forster R., Grandy N., Huet M-C., Lewis G., Oomen P.A., Schmuck R. & Vogt H., 2001. Guidance document on regulatory testing and risk assessment procedures for plant protection products with non-target arthropods. From the ESCORT 2 workshop (European Standard Characteristics Of non-target arthropods Regulatory Testing), a joint BART, EOOP/CoE, OECD and IOBC Workshop organized in conjunction with SETAC-Europe and EC. SETAC Europe publisher, 2001
- Heimbach, U., Wehling A., Candolfi, M., Coulson, M., Halsall, N., Jäckel, B., Mead-Briggs, M., Nienstedt, K., Römbke, J., Schmitzer, S., Schmuck, R., Ufer, A., Wilhelmy, H. (2000): A method for testing effects of plant protection products on spiders of the genus Pardosa (Araneae, Lycosidae) under laboratory conditions.