- Aquatic Ecotoxicology
- OECD 202: Daphnia sp., Acute Immobilisation Test
- OECD 211: Daphnia magna Reproduction Test
- OECD 235: Chironomus sp., Acute Immobilisation Test
- OECD 218/219: Sediment-Water Chironomid Toxicity Test Using Spiked Sediment/Spiked Water
- OECD 233: Sediment-Water Chironomid Life-Cycle Toxicity Test Using Spiked Water or Spiked Sediment
- OECD 225: Sediment-water Lumbriculus Toxicity Test Using Spiked Sediment
- OECD 242: Potamopyrgus antipodarum Reproduction Test
- OECD 243: Lymnea stagnalis Reproduction Test
- OECD 203: Fish, Acute Toxicity Test
- OECD 215: Fish Juvenile Growth Study
- OECD 212: Fish, Short-term Toxicity Test on Embryo and Sac-fry Stages
- OECD 231: The Amphibian Metamorphosis Assay
- OECD 236: Fish Embryo Acute Toxicity Test
- OECD 210: Fish, Early-life Stage Toxicity Test
- OECD 229 Fish Short Term Reproduction Assay and OECD 230 21-day Fish Assay
- OECD 240 Medaka Extended One Generation Reproduction Test (MEOGRT)
- OECD 241: Larval Amphibian Growth and Development Assay
- OPPTS 850.1500: Fish Life Cycle Toxicity Test
- OÈCD 234 Fish sexual development test
- Storage Stability Studies
- OPPTS 830.6302, OPPTS 830.6303,and OPPTS 830.6304: Physical State, Colour and Odor at 20 °C and at 101.3 kPa
- EU A.1: Melting temperature/range
- EU A.2: Boiling temperature
- EU A.3: Relative density (liquids and solids)
- EU A.4: Vapour pressure
- EU A.5: Surface tension
- EU A.9: Flashpoint
- EU A.10: Flammability (solids)
- EU A.12: Flammability (contact with water)
- EU A.13: Pyrophoric properties of solids and liquids
- EU A.16: Relative self-ignition temperature for solids
- EU A.17: Oxidising properties
- OECD 114: Viscosity of Liquids
- Environmental Fate
- Terrestrial Ecotoxicology
- Non-target arthropod testing with the parasitic wasp (Aphidius rhopalosiphi)
- Non-target arthropod testing with the lacewing (Chrysoperla carnea)
- Non-target arthropod testing with the ladybird beetle (Coccinella septempunctata)
- Non-target arthropod testing with the predatory bug (Orius laevigatus)
- Non-target arthropod testing with the predatory mite (Typhlodromus pyri)
- Non-target arthropod testing with the rove beetle (Aleochara bilineata)
- Non-target arthropod testing with the carabid beetle (Poecilus cupreus)
- Non-target arthropod testing with the wolf spider (Pardosa spec.)
- OECD 213/214: Honey bees, Acute Oral and Acute Contact Toxicity Test
- OECD 245: Honey Bee (Apis Mellifera L.), Chronic Oral Toxicity Test (10-Day Feeding)
- OECD 237: Honey Bee Larval Toxicity Test, Single Exposure
- OECD 239: Honey Bee Larval Toxicity Test
- EPPO 170: Honey Bee Field Study – do plant protection products effect honey bee colonies?
- Oomen et al. 1992: Honey Bee Brood Feeding Study
- OECD 75: Honey Bee Brood Test under Semi-field Conditions in Tunnels
- OECD 246/247 Acute Oral and Contact Toxicity to the Bumblebee, Bombus terrestris L.
- Solitary Bee Acute Contact Toxicity Study in the Laboratory (Osmia sp.) Solitary Bee Acute Oral Toxicity Study in the Laboratory (Osmia sp.) (protocols for ringtests with solitary bees recommended by the non-Apis working group)
- SANTE/11956/2016 rev.9 Residue trials for MRL setting in honey
- OECD 208: Terrestrial Plant Test - Seedling Emergence and Seedling Growth Test
- OECD 227: Terrestrial Plant Test - Vegetative Vigour Test
- OCSPP 850.4100: Seedling Emergence and Seedling Growth
- OCSPP 850.4150: Vegetative Vigor
- EPPO PP 1/207(2): Efficacy evaluation of plant protection products, Effects on succeeding crops
- Ecological Modelling
- Quality Assurance
- Testing of Potential Endocrine Disruptors
- Aquatic Ecotoxicology
- Who we are
- Company history
- Our Certificates
Water-sediment Glyceria maxima toxicity test
The EFSA Guidance on tiered risk assessment for edge-of-field surface-waters (2013) necessitates the testing of Glyceria maxima for special cases. This study determines the effects of a test item on the vegetative growth or increase in biomass of G. maxima. Despite the fact that G. maxima testing is quite a new requirement, ibacon has already performed many such studies and is experienced with this species. Aquatic plants found in static water bodies may experience prolonged or continuous exposure to chemicals as well as repeated exposures. Therefore we offer not only the required dose-response design, but also recovery designs with the ability for the plants to recover from the treatments and pulsed exposures . These scenarios can be simulated by static or static renewal systems.
The reed sweet grass G. maxima grow as an emergent monocotyledon. It is found as a pure, dense colony in permanently or seasonally flooded areas over nutrient-rich substrates. New plants develop predominantly by vegetative spread of rhizomes. However, these plants are not easy to cultivate, but we manage to do it in our laboratory and therefore we are not dependent on the delivery from commercial breeders. Our plants are free from parasites and we are sure that they are completely untreated.
Course of the test
Essentially a ring-test is conducted in order to develop a guideline for G. maxima testing. The general procedure is comparable to the water-sediment toxicity test with Myriophyllum spicatum.
Before test start the plants have to be kept under test conditions (16:8 light/dark ratio, high light intensity, 23 ± 2°C) to adapt the plants. One single plant is introduced into a small pot containing artificial sediment and is exposed in a vessel with reconstituted test media (Smart and Barko). The test medium should cover the sediment surface by at least 3 cm. Each test should consist of a geometric series of test concentrations with four to five replicates and an untreated control with six to eight replicates.
After the acclimatization phase plants with 1-2 unfolded leaves can be used as test plants. The test substance is applied via the test media or via the artificial sediment and during the 14 - 21 days of exposure normally no water exchange is conducted. If the test item degrades fast a semi-static approach can be conducted.
At test start and test termination shoot height, total leaf lenght, fresh weight and dry weight are determined.
The endpoints of the study are the increase in shoot height (including newly developed shoots) as well as fresh and dry weight. Based on these parameters NOEC, LOEC and EC50 (EC20, EC10) can be calculated for yield and growth rate. Moreover sublethal effects, such as growth abnormalities or necrosis are determined.
Guidelines and Literature
- EFSA Guidance on tiered risk assessment for plant protection products for aquatic organisms in edge-of-field surface waters. EFSA Journal 2013; 11 (7): 3290.
Ring-test protocol for evaluating the effects of Imazapyr on Glyceria maxima in a water-sediment system, July 2018.