- Aquatic Ecotoxicology
- OECD 202: Daphnia sp., Acute Immobilisation Test
- OECD 211: Daphnia magna Reproduction Test
- OECD 235: Chironomus sp., Acute Immobilisation Test
- OECD 218/219: Sediment-Water Chironomid Toxicity Test Using Spiked Sediment/Spiked Water
- OECD 233: Sediment-Water Chironomid Life-Cycle Toxicity Test Using Spiked Water or Spiked Sediment
- OECD 225: Sediment-water Lumbriculus Toxicity Test Using Spiked Sediment
- OECD 242: Potamopyrgus antipodarum Reproduction Test
- OECD 243: Lymnea stagnalis Reproduction Test
- OECD 203: Fish, Acute Toxicity Test
- OECD 215: Fish Juvenile Growth Study
- OECD 212: Fish, Short-term Toxicity Test on Embryo and Sac-fry Stages
- OECD 231: The Amphibian Metamorphosis Assay
- OECD 236: Fish Embryo Acute Toxicity Test
- OECD 210: Fish, Early-life Stage Toxicity Test
- OECD 229 Fish Short Term Reproduction Assay and OECD 230 21-day Fish Assay
- OECD 240 Medaka Extended One Generation Reproduction Test (MEOGRT)
- OECD 241: Larval Amphibian Growth and Development Assay
- OPPTS 850.1500: Fish Life Cycle Toxicity Test
- OÈCD 234 Fish sexual development test
- Storage Stability Studies
- OPPTS 830.6302, OPPTS 830.6303,and OPPTS 830.6304: Physical State, Colour and Odor at 20 °C and at 101.3 kPa
- EU A.1: Melting temperature/range
- EU A.2: Boiling temperature
- EU A.3: Relative density (liquids and solids)
- EU A.4: Vapour pressure
- EU A.5: Surface tension
- EU A.9: Flashpoint
- EU A.10: Flammability (solids)
- EU A.12: Flammability (contact with water)
- EU A.13: Pyrophoric properties of solids and liquids
- EU A.16: Relative self-ignition temperature for solids
- EU A.17: Oxidising properties
- OECD 114: Viscosity of Liquids
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- Terrestrial Ecotoxicology
- Non-target arthropod testing with the parasitic wasp (Aphidius rhopalosiphi)
- Non-target arthropod testing with the lacewing (Chrysoperla carnea)
- Non-target arthropod testing with the ladybird beetle (Coccinella septempunctata)
- Non-target arthropod testing with the predatory bug (Orius laevigatus)
- Non-target arthropod testing with the predatory mite (Typhlodromus pyri)
- Non-target arthropod testing with the rove beetle (Aleochara bilineata)
- Non-target arthropod testing with the carabid beetle (Poecilus cupreus)
- Non-target arthropod testing with the wolf spider (Pardosa spec.)
- OECD 213/214: Honey bees, Acute Oral and Acute Contact Toxicity Test
- Honeybee: Chronic Feeding Tests
- OECD 237: Honey Bee Larval Toxicity Test, Single Exposure
- Effects on Honey Bee Brood (Apis mellifera) - Brood Feeding Test -
- OECD Draft TG: Honey Bee Larval Toxicity Test, Repeated Exposure
- OECD 75: Honey Bee Brood Test under Semi-field conditions
- EPPO 170: Honey Bee Field Study – do plant protection products effect honey bee colonies?
- Acute Oral and Contact Toxicity to the Bumblebee, Bombus terrestris L.
- Ecological Modelling
- Quality Assurance
- Testing of Potential Endocrine Disruptors
- Aquatic Ecotoxicology
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EU A.5: Surface tension
The purpose of the study is to evaluate the surface tension of an aqueous solution by means of a ring tensiometer.
It is useful to have preliminary information on the water solubility of the substance before performing these tests.
(If the water solubility is below 1 mg/l at 20°C the test does not need to be conducted).
Course of the test
Apparatus - Tensiometer (model K8, KRÜSS)
Test Condition - 20 °C ± 0.5 °C
According to the guideline the concentration to use is depending on the water solubility of the test item.
The test concentration should be 90 % of the saturation solubility in case the solubility value is below 1 g/L. Otherwise 1g/L will be used as test concentration.
The cleaned measurement vessel will be filled with the test item solution and placed into the mobile sample table. The table will be raised until the ring is immersed below the surface of the solution. Subsequently, the table will be lowered until the ring is attached to the liquid surface. After additional lowering of the table, the ring exerts a torque on the force measuring system. Subsequently, the table top should be lowered gradually and evenly at a maximum rate of 0.5 cm/min to detach the ring from the surface. Lowering of the table and subsequent compensation of the resulting torque are continued in small increments until the maximum force is reached and the lamina breaks. This is when upward force completely overcomes the surface tension force and the ring is torn away from the surface. After completing the measurement, the ring will be immersed below the surface again and the measurement will be repeated at least 5 times until a constant value is reached. The time passed since the solution was transferred to the measurement vessel will be recorded for each determination.
The surface tension at 20 °C will be reported as mN/m (SI sub-unit) or dyn/cm (CGS system).
Guidelines and Literature
- Commission Regulation (EC) No 440/2008 laying down test methods pursuant to Regulation (EC) No 1907/2006 of the European Parliament and of the Council on the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) (originally published as Council Regulation (EC) No 440/2008, corrigendum according to Official Journal of the European Union (EN) dated June 3, 2008, L 143/55), Annex, Part A, A.5.: “Surface Tension”, Official Journal of the European Union, L 142, Volume 51, May, 31 2008
- Organisation for Economic Co-operation and Development (OECD), Guidelines for Testing of Chemicals, Guideline No.: 115, “Surface Tension of Aqueous Solutions”, adopted October 02, 2012