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Xenopus Embryonic Thyroid Assay in co-operation with Laboratoire Watchfrog

31st July 2019

Amphibian metamorphosis is controlled by thyroid hormones and is associated with TH/bZIP gene expression. This process can be made visible through use of the "Green Fluorescent Protein" (GFP); exhibiting bright green fluorescence under TH/bZIP gene expression. GFP incorporated into the genome of the african clawed frog (Xenopus laevis) is heritable and thus allows to obtain translucent aquatic larvae able to express GFP. Measuring the intensity of the fluorescence of GFP allows the quantification of thyroid disruption induced by endocrine disruptors. This process is the basis for the Xenopus Eleutheroembryo Thyroid Assay (XETA); an in vitro assay implemented in the OECD Conceptual Framework for the Screening and Testing of Endocrine Disrupting Chemicals (level 3) and described in the ECHA/EFSA guidance document to identify endocrine active chemicals.

The main advantage of these tests is, that it takes into account the entire physiological pathway leading to disruption and could be interpreted as specific endocrine mechanism.

Supported by our partner Laboratoire Watchfrog ibacon can now offer the XETA OECD 248 test under GLP. The partnership between Laboratoire Watchfrog and Ibacon was selected in order to offer this valuable scientific technology for GLP compliant studies on ED properties in the regulatory context for Plant Protection Products, Biocides, Industrial Chemicals, Pharmaceuticals and Veterinary Medicinal Products. Please contact us for further questions and availability.

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