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Nondestructive sampling of vitellogenin from skin mucus of fish
31st January 2018 - 15th March 2018
The identification of endocrine disruptors is an important concern of chemical risk assessment. In the presense of estrogenic endocrine disrupters the biomarker vitellogenin is expressed in male and immature fish. Vitellogenin is present e.g. in blood, hemolymphe, liver and epidermis of fish and other egg laying vertebrates. Allner et al. 2016 compared the vitellogenin content in different tissues of cyprinid and perciform fishes and evaluated sensitive enzyme-linked immunosorbent assays. The vitellogenin content in the skin mucus of juvenile and male fish after exposure to estradiol and bisphenol A was comparable to the contents in fish blood.
The determination of vitellogenin in swab samples of fish skin mucus offers the possibility to assess living fish - either in the frame of OECD guidelines for testing endocrine disruption or for monitoring purposes in the environment. Therefore it is not only a simple sampling procedure but also an improvement in the sense of animal welfare.
ibacon conducted the analytical investigations of estradiol and bisphenol A. Our colleague Marco Willner set up an LC-MS/MS method.
You are interested to know more about the details? Please have a look at the publication.
Allner, Hennies, Lerche, Schmidt, Schneider, Willner, Stahlschmidt-Allner 2016, Kinetic determination of vitellogenin induction in the epidermis of cyprinid and perciform fishes: Evaluation of sensitive enzyme-linked immunosorbent assays, Environmental Toxicology and Chemistry, Vol. 35 No.12, pp. 2916 - 2930, 2016.